Human NRG1-beta 1/HRG1-beta 1 DuoSet ELISA

R&D Systems, part of Bio-Techne | Catalog # DY377

R&D Systems, part of Bio-Techne
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DY377
DY377-05
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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

125-4000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human NRG1-beta 1/HRG1-beta 1 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

View all Neuregulin-1 beta 1/NRG1 beta 1 ELISA Kits »

Product Summary for Human NRG1-beta 1/HRG1-beta 1 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human NRG1-beta 1/HRG1-beta 1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human NRG1-beta 1/HRG1-beta 1 DuoSet ELISA

Human Neuregulin-1 beta 1 / NRG1 beta 1 ELISA Standard Curve

Human Neuregulin-1 beta 1 / NRG1 beta 1 ELISA Standard Curve

Kit Contents for Human NRG1-beta 1/HRG1-beta 1 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

Normal Goat Serum: (Catalog # DY005)

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Normal Goat Serum: (Catalog # DY005)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Neuregulin-1 beta 1/NRG1 beta 1

The neuregulin (also known as heregulin) cytokine family is comprised of four genes that encode a large number of secreted or membrane-bound isoforms. All family members share an EGF-like domain that interacts with the ErbB family of tyrosine kinase receptors. The NRG1 gene is the most pleiotropic, encoding more than 14 soluble or transmembrane proteins. NRG1 isoforms can be classified into type I (neu differentiation factor, NDF; heregulin, HRG; acetylcholine receptor inducing activity, ARIA), type II (glial growth factor, GGF) and type III (sensory and motor neuron-derived factor, SMDF) isoforms. NRG proteins show distinct spatial and temporal expression patterns and play important roles during development of both the nervous system and the heart.

Alternate Names

Neuregulin1 beta 1

Entrez Gene IDs

3084 (Human); 211323 (Mouse); 112400 (Rat)

Gene Symbol

NRG1

Additional Neuregulin-1 beta 1/NRG1 beta 1 Products

Product Documents for Human NRG1-beta 1/HRG1-beta 1 DuoSet ELISA

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NRG1-beta 1/HRG1-beta 1 DuoSet ELISA

For research use only

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