Human Phospho-PRAS40 (T246) DuoSet IC ELISA

R&D Systems, part of Bio-Techne | Catalog # DYC6890-2

R&D Systems, part of Bio-Techne
Discontinued Product
DYC6890-2 has been discontinued. View all PRAS40 products.

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

937.5-60000 pg/mL

Sample Type

Cell Lysates

Reactivity

Human

Human Phospho-PRAS40 (T246) DuoSet IC ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

View all PRAS40 ELISA Kits »

Product Summary for Human Phospho-PRAS40 (T246) DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure phosphorylated in cell lysates. An immobilized capture antibody specific for binds both phosphorylated and unphosphorylated . After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

Cell lysates (100 µL)

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Kit Contents for Human Phospho-PRAS40 (T246) DuoSet IC ELISA

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PRAS40

PRAS40 (40 kDa proline-rich Akt1 substrate), also known as AKT1S1, is a widely expressed component of the cytoplasmic mTOR complex (mTORC1). In response to insulin stimulation, Akt1 phosphorylation of PRAS40 relieves the basal PRAS40-mediated inhibition of mTORC1. Activated mTORC1 is a key effector of the cell survival pathway. PRAS40 phosphorylation by Akt3 or Pim1 similarly exerts an anti-apoptotic effect during tumor progression and neuronal ischemia . Alternate splicing generates an isoform that lacks the N-terminal 130 amino acids.

Long Name

40 kDa Proline-rich Akt1 Substrate

Alternate Names

AKT1S1, Lobe

Entrez Gene IDs

84335 (Human); 67605 (Mouse); 292887 (Rat)

Gene Symbol

AKT1S1

Additional PRAS40 Products

Product Documents for Human Phospho-PRAS40 (T246) DuoSet IC ELISA

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Phospho-PRAS40 (T246) DuoSet IC ELISA

For research use only

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