Does the Human PlGF Quantikine® ELISA Kit, Catalog # DPG00, detect free or total PlGF?

Name: Human PlGF Quantikine ELISA Kit
Brand: R&D Systems
SKU: DPG00
Rating: 5 (3 reviews)

Soluble receptors or other binding proteins present in biological samples do not necessarily interfere with the measurement of ligands in samples. However, until the factors have been tested in the Quantikine® Immunoassay, the possibility of interference cannot be excluded. We cannot claim Catalog # DPG00 to be a "free" or "total" PlGF detecting kit, since all possible binding factors have not been tested. Specificity testing performed using rhFlt-1/Fc (VEGF R1) for interference demonstrated interference at levels greater than 2000 pg/mL. Details of this testing can be found in the DPG00 product insert. Most samples will have lower levels of sFlt-1 than 2000 pg/ml.

Human PlGF Quantikine ELISA Kit

R&D Systems, part of Bio-Techne | Catalog # DPG00

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Citations (120)

Product Datasheet / COA / SDS

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Product Summary
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Length

3.5 hours or 4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL), Urine (100 µL)

Sensitivity

7 pg/mL

Assay Range

15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)

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Product Summary for Human PlGF Quantikine ELISA Kit

The Quantikine Human PlGF Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure human PlGF in cell culture supernates, serum, plasma, and urine. The immunoassay kit contains E. coli-expressed recombinant human PlGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human PlGF accurately. Results obtained using natural human PlGF showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PlGF.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Human

Specificity

Natural and recombinant human PlGF

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity not tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	40.9	138	541	46.9	149	555
Standard Deviation	2.4	7.3	38.2	6.4	13.5	32.9
CV%	5.9	5.3	7.1	13.6	9.1	5.9

Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	54.3	175	658	55.0	184	724
Standard Deviation	3.8	6.3	36.9	6.5	20.3	78.9
CV%	7.0	3.6	5.6	11.8	11.0	10.9

Recovery for Human PlGF Quantikine ELISA Kit

The recovery of PlGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	97	82-107
Citrate Plasma (n=5)	99	93-120
EDTA Plasma (n=5)	102	92-117
Heparin Plasma (n=5)	94	81-113
Serum (n=5)	96	88-118
Urine (n=4)	97	85-112

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of PlGF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Images for Human PlGF Quantikine ELISA Kit

Human PlGF ELISA Cell Culture Supernate Standard Curve

Human PlGF ELISA Serum/Plasma/Urine Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PlGF

PlGF (placenta growth factor) is a homodimeric growth factor that competes with VEGF for binding to VEGF R1/Flt-1. It therefore increases the availability of VEGF to bind to VEGF R2/KDR/Flk-1 and trigger angiogenesis. It can form heterodimers with some forms of VEGF and decrease the angiogenic effect of VEGF on VEGF R2. Circulating PlGF levels increase during pregnancy, reaching a peak in mid-gestation; this increase is attenuated in preeclampsia. PlGF induces monocyte activation and migration as well as production of inflammatory cytokines and VEGF. These activities facilitate wound, bone fracture, and cardiac repair, but also contribute to inflammation in active sickle cell disease and atherosclerosis. PlGF can also inhibit TIMP3 expression in the spleen, leading to immune triggering of hypertension.

Long Name

Placenta Growth Factor

Alternate Names

PGF, PGFL

Entrez Gene IDs

5228 (Human); 18654 (Mouse)

Gene Symbol

PGF

Additional PlGF Products

Product Documents for Human PlGF Quantikine ELISA Kit

Product Datasheets

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COA

SDS

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Product Specific Notices for Human PlGF Quantikine ELISA Kit

For research use only

Citations
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.