Human Pro-Cathepsin B Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DCATB0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (25 µL), Serum (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (25 µL)
Sensitivity
0.079 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, Heparin Plasma, Saliva, Urine)
Product Summary for Human Pro-Cathepsin B Quantikine ELISA Kit
The Quantikine Human Pro-Cathepsin B Immunoassay is a 4.5 hour solid phase ELISA designed to measure the pro-form of cathepsin B in cell culture supernates, cell lysates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human Pro-Cathepsin B and antibodies raised against the recombinant factor. Natural human Pro-Cathepsin B showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human Pro-Cathepsin B.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Pro-Cathepsin B
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 1.08 | 2.39 | 4.50 | 1.16 | 2.58 | 4.91 |
| Standard Deviation | 0.05 | 0.11 | 0.23 | 0.10 | 0.16 | 0.30 |
| CV% | 4.6 | 4.6 | 5.1 | 8.6 | 6.2 | 6.1 |
Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 1.32 | 2.93 | 5.78 | 1.48 | 3.18 | 5.95 |
| Standard Deviation | 0.08 | 0.15 | 0.29 | 0.13 | 0.24 | 0.42 |
| CV% | 6.1 | 5.1 | 5.0 | 8.8 | 7.5 | 7.1 |
Recovery for Human Pro-Cathepsin B Quantikine ELISA Kit
The recovery of Pro-Cathepsin B spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 106 | 98-115 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of Pro-Cathepsin B were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Pro-Cathepsin B Quantikine ELISA Kit
Human Pro-Cathepsin B ELISA Serum/Plasma Standard Curve
Human Pro-Cathepsin B ELISA Cell Culture Supernate/Cell Lysate/Saliva/Urine Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Cathepsin B
Cathepsin B is the first described member of the family of lysosomal cysteine proteases. Cathepsin B possesses both endopeptidase and exopeptidase activities, in the latter case acting as a peptidyl-dipeptidase. It is known to process a number of proteins, including pro and active caspases, prorenin and SLPI. Cathepsin B is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme.
Additional Cathepsin B Products
Product Documents for Human Pro-Cathepsin B Quantikine ELISA Kit
Product Specific Notices for Human Pro-Cathepsin B Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.