Human Pro-Collagen I alpha 1 ELISA Kit - Quantikine
R&D Systems, part of Bio-Techne | Catalog # DPCA00
Key Product Details
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 µL), Cell Lysates (25 µL), Tissue Lysates (25 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (25 µL)
Sensitivity
8.75 pg/mL
Assay Range
78.1-5000 pg/mL (Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Product Summary for Human Pro-Collagen I alpha 1 Quantikine ELISA Kit
The Quantikine Human Pro-Collagen I alpha 1/COLIA1 (Pro-Collagen I alpha1) Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human Pro-Collagen I alpha1 in cell culture supernates, cell lysates, tissue extracts, serum, plasma, saliva, and urine. It contains recombinant human Pro-Collagen I alpha1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Pro-Collagen I alpha1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Pro-Collagen I alpha1.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Pro-Collagen I alpha1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Saliva, Serum, Tissue Lysates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 442 | 1410 | 2669 | 448 | 1378 | 2677 |
| Standard Deviation | 17.8 | 32.4 | 50.1 | 28.9 | 49.8 | 109 |
| CV% | 4.0 | 2.3 | 1.9 | 6.4 | 3.6 | 4.1 |
Recovery for Human Pro-Collagen I alpha 1 Quantikine ELISA Kit
The recovery of human Pro-Collagen I alpha1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Extraction Buffer (n=1) | 105 | 101-110 |
| Lysis Buffer (n=1) | 97 | 94-101 |
| Media (n=4) | 107 | 97-118 |
| Saliva (n=4) | 97 | 87-114 |
| Urine (n=4) | 90 | 79-111 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of human Pro-Collagen I alpha1 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Pro-Collagen I alpha 1 Quantikine ELISA Kit
Human Pro-Collagen I alpha 1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Collagen I alpha 1
Additional Collagen I alpha 1 Products
Product Documents for Human Pro-Collagen I alpha 1 Quantikine ELISA Kit
Product Specific Notices for Human Pro-Collagen I alpha 1 Quantikine ELISA Kit
For research use only
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