Human Prolactin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DPRL00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
0.264 ng/mL
Assay Range
1.56-100 ng/mL (Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human Prolactin Quantikine ELISA Kit
The Quantikine Human Prolactin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Prolactin in serum and plasma. It contains E. coli-expressed recombinant human Prolactin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Prolactin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Prolactin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Prolactin and natural human macroprolactin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 16.3 | 31.8 | 53.1 | 15.4 | 30.6 | 54.4 |
| Standard Deviation | 0.831 | 1.24 | 1.79 | 0.90 | 1.48 | 2.94 |
| CV% | 5.1 | 3.9 | 3.4 | 5.8 | 4.8 | 5.4 |
Recovery for Human Prolactin Quantikine ELISA Kit
The recovery of Prolactin spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 94 | 84-105 |
| Heparin Plasma (n=4) | 97 | 90-104 |
| Serum (n=4) | 94 | 84-108 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Prolactin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Prolactin Quantikine ELISA Kit
Human Prolactin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Prolactin
Prolactin receptor (PRL R) contains an extracellular, transmembrane, and intracellular domain. Transcriptional regulation of the PRL R gene results in several different species-dependent isoforms of PRL R being produced.
Additional Prolactin Products
Product Documents for Human Prolactin Quantikine ELISA Kit
Product Specific Notices for Human Prolactin Quantikine ELISA Kit
For research use only
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.