Human PSGL-1/CD162 DuoSet ELISA

Key Product Details

Product Specifications

Kit Contents for Human PSGL-1/CD162 DuoSet ELISA

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween^® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Preparation and Storage

Background: PSGL-1/CD162

PSGL-1 (P-Selectin Glycoprotein Ligand 1) is type I transmembrane receptor found on the surface of neutrophils, monocytes, and most lymphocytes. It is expressed as a dimeric mucin-like glycoprotein that is N-glycosylated and contains both sialylated and fucosylated O-linked oligosaccharides. PSGL-1 binds to P-, E- and L-Selectins. The calcium-dependent high affinity interaction with P-, E-, and L-Selectin mediates the tethering and rolling of neutrophils, monocytes, and/or lymphocytes on endothelial cells.

Additional PSGL-1/CD162 Products

• All Products for PSGL-1/CD162
• PSGL-1/CD162 ELISA Kits
• PSGL-1/CD162 Lysates
• PSGL-1/CD162 Primary Antibodies
• PSGL-1/CD162 Proteins and Enzymes