Human RBP4 Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DRB400
Key Product Details
Assay Length
2.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (20 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (10 µL)
Sensitivity
0.628 ng/mL
Assay Range
1.56-100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Product Summary for Human RBP4 Quantikine ELISA Kit
The Quantikine Human RBP4 immunoassay is a 2.5 hour solid phase ELISA designed to measure human RBP4 in cell culture supernates, serum, plasma, urine, and saliva. It contains NS0-expressed recombinant human RBP4 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human RBP4 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human RBP4.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human RBP4
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma/Saliva/Urine - Samples from apparently healthy volunteers were evaluated for
the presence of human RBP4 in this assay. No medical histories were available for the donors
used in this study.
Cell Culture Supernates:
| Sample Type | Mean (pg/mL) | Range (pg/mL) | Standard Deviation (pg/mL) |
| Serum (n=35) | 26,500 | 12,700-48,600 | 7700 |
| EDTA plasma (n=35) | 25,200 | 11,900-48,300 | 7200 |
| Heparin plasma (n=35) | 25,000 | 12,200-43,000 | 7500 |
| Urine (n=11) | 85.2 | 16.4-252 | 66.8 |
| Salive (n=12) | 14.9 | 3.11-29.6 | 10.0 |
Cell Culture Supernates:
Human peripheral blood mononuclear cells (1 x 106
cells/mL) were cultured in DMEM
supplemented with 5% fetal bovine serum, 5 μM beta-mercaptoethanol, 2 mM L-glutamine,
100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or
stimulated with 10 μg/mL PHA for 1 and 5 days. Aliquots of the cell culture supernates were
removed and assayed for levels of human RBP4. No detectable levels were observed.
HepG2 human hepatocellular carcinoma cells (1 x 106
cells/mL) were cultured in MEM
supplemented with non-essential amino acids, 10% fetal bovine serum, and 2 mM L-glutamine
for 5 days. An aliquot of the cell culture supernate was removed, assayed for human RBP4, and
measured 75.2 ng/mL.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 11.0 | 30.3 | 61.9 | 9.80 | 27.5 | 54.7 |
| Standard Deviation | 0.77 | 1.73 | 5.01 | 0.84 | 1.60 | 4.00 |
| CV% | 7.0 | 5.7 | 8.1 | 8.6 | 5.8 | 7.3 |
Recovery for Human RBP4 Quantikine ELISA Kit
The recovery of RBP4 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 86-115 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of RBP4 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human RBP4 Quantikine ELISA Kit
Human RBP4/Retinol-Binding Protein 4 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: RBP4/Retinol-Binding Protein 4
Alternate Names
RetinolBinding Protein 4
Gene Symbol
RBP4
Additional RBP4/Retinol-Binding Protein 4 Products
Product Documents for Human RBP4 Quantikine ELISA Kit
Product Specific Notices for Human RBP4 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.