Human Serpin G1/C1 Inhibitor Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DSGC10
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (10 µL), Human Milk (10 µL)
Sensitivity
25.6 pg/mL
Assay Range
62.5-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Product Summary for Human Serpin G1/C1 Inhibitor Quantikine ELISA Kit
The Quantikine Human Serpin G1/C1 Inhibitor Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Serpin G1 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains human Serpin G1 purified from plasma and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Serpin G1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Serpin G1.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural human Serpin G1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
| Sample Type | Mean (ng/mL) | Range (ng/mL) |
| Serum (n=30) | 138,746 | 55,639-323,345 |
| EDTA Plasma (n=30) | 136,446 | 76,442-259,277 |
| Heparin (n=30) | 131,218 | 77,947-225,264 |
| Saliva (n=10) | 50.5 | 11.0-121 |
| Urine (n=10) | 66.1 | 16.9-176 |
| Human Milk (n=10) | 601 | 238-1318 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Human Milk, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 206 | 634 | 1209 | 212 | 603 | 1191 |
| Standard Deviation | 10.4 | 24.7 | 56.5 | 13.7 | 31.7 | 53.4 |
| CV% | 5.0 | 3.9 | 4.7 | 6.5 | 5.3 | 4.5 |
Recovery for Human Serpin G1/C1 Inhibitor Quantikine ELISA Kit
The recovery of human Serpin G1 spiked to levels throughout the range of the assay in cell culture media was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 100 | 90-110 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Serpin G1 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Serpin G1/C1 Inhibitor Quantikine ELISA Kit
Human Serpin G1/C1 Inhibitor ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Serpin G1/C1 Inhibitor
Additional Serpin G1/C1 Inhibitor Products
Product Documents for Human Serpin G1/C1 Inhibitor Quantikine ELISA Kit
Product Specific Notices for Human Serpin G1/C1 Inhibitor Quantikine ELISA Kit
For research use only
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