Human SLPI Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DPI00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL), Urine (20 µL)
Sensitivity
25 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Product Summary for Human SLPI Quantikine ELISA Kit
The Quantikine Human SLPI Immunoassay is a 4.5 hour solid phase ELISA designed to measure SLPI in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human SLPI and antibodies raised against the recombinant factor. The kit has been shown to accurately quantitate recombinant human SLPI. Results obtained using natural human SLPI showed linear curves that were parallel to the curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human SLPI.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human SLPI
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 109 | 693 | 2026 | 188 | 1174 | 2157 |
| Standard Deviation | 8.7 | 37.1 | 84.1 | 15.1 | 57.5 | 123 |
| CV% | 8.0 | 5.4 | 4.2 | 8.0 | 4.9 | 5.7 |
Recovery for Human SLPI Quantikine ELISA Kit
The recovery of SLPI was determined in various matrices by mixing high dose samples with low dose samples in ratios of 1:2, 1:1, and 2:1.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 94 | 84-105 |
| Citrate Plasma (n=10) | 97 | 92-102 |
| EDTA Plasma (n=10) | 96 | 87-103 |
| Heparin Plasma (n=10) | 106 | 98-116 |
| Serum (n=10) | 98 | 89-104 |
| Urine (n=25) | 98 | 92-103 |
Linearity
To assess the linearity of the assay, the following biological samples containing, or spiked with high concentrations of SLPI, were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Human SLPI Quantikine ELISA Kit
Human SLPI ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: SLPI
Long Name
Secretory Leukocyte Protease Inhibitor
Alternate Names
ALK1, ALPProtease inhibitor WAP4, antileukoproteinase, BLPISeminal proteinase inhibitor, HUSI, HUSI-I, Mucus proteinase inhibitor, secretory leukocyte peptidase inhibitor, Secretory leukocyte protease inhibitor, secretory leukocyte protease inhibitor (antileukoproteinase), WAP four-disulfide core domain protein 4, WAP4HUSI-1, WFDC4MPI
Gene Symbol
SLPI
Additional SLPI Products
Product Documents for Human SLPI Quantikine ELISA Kit
Product Specific Notices for Human SLPI Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.