Human SOST/Sclerostin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DSST00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
3.8 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human SOST/Sclerostin Quantikine ELISA Kit
The Quantikine Human SOST immunoassay is a 4.5 hour solid-phase
ELISA designed to measure SOST in human cell culture supernates,
serum, and plasma. It contains NS0-expressed recombinant human SOST and
antibodies raised against the recombinant factor. This immunoassay has
been shown to accurately quantitate the recombinant human SOST. Results
obtained using natural human SOST showed dose response curves
that were parallel to the standard curves obtained using the Quantikine
kit standards. These results indicate that this kit can be used to
determine relative mass values for natural SOST.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human SOST
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 308 | 577 | 1208 | 315 | 591 | 1234 |
| Standard Deviation | 6.03 | 10.5 | 25.1 | 25.9 | 63.8 | 118 |
| CV% | 2.0 | 1.8 | 2.1 | 8.2 | 10.8 | 9.6 |
Recovery for Human SOST/Sclerostin Quantikine ELISA Kit
The recovery of SOST spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 100 | 91-111 |
| EDTA Plasma (n=4) | 95 | 89-101 |
| Heparin Plasma (n=4) | 100 | 91-108 |
| Serum (n=4) | 96 | 86-112 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human SOST were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human SOST/Sclerostin Quantikine ELISA Kit
Human SOST/Sclerostin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: SOST/Sclerostin
Alternate Names
sclerostin, VBCHsclerosteosis
Gene Symbol
SOST
Additional SOST/Sclerostin Products
Product Documents for Human SOST/Sclerostin Quantikine ELISA Kit
Product Specific Notices for Human SOST/Sclerostin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.