Human SP-D Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DSFPD0
Key Product Details
Assay Length
5.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
0.37 ng/mL
Assay Range
0.625-40 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human SP-D Quantikine ELISA Kit
The Quantikine Human SP-D Immunoassay is a 5.5 hour solid-phase ELISA designed to measure human SP-D in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human SP-D and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human SP-D showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally ocurring SP-D.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human SP-D
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 6.07 | 11.7 | 23.4 | 5.63 | 11.2 | 22.8 |
| Standard Deviation | 0.495 | 0.588 | 1.46 | 0.495 | 0.999 | 1.78 |
| CV% | 8.2 | 5.0 | 6.2 | 8.8 | 8.9 | 7.8 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 5.99 | 11.3 | 24.3 | 6.63 | 13.1 | 26.9 |
| Standard Deviation | 0.371 | 0.921 | 1.63 | 0.617 | 1.14 | 2.50 |
| CV% | 6.2 | 8.2 | 6.7 | 9.3 | 8.7 | 9.3 |
Recovery for Human SP-D Quantikine ELISA Kit
The recovery of SP-D spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 95-109 |
| EDTA Plasma (n=4) | 110 | 99-120 |
| Heparin Plasma (n=4) | 104 | 85-118 |
| Serum (n=4) | 106 | 89-118 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SP-D were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human SP-D Quantikine ELISA Kit
Human SP-D ELISA Cell Culture Supernate Standard Curve
Human SP-D ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: SP-D
Long Name
Surfactant Pulmonary Associated Protein D
Alternate Names
Collectin 7, PSP-D, SFTP4, SFTPD, SPD
Gene Symbol
SFTPD
Additional SP-D Products
Product Documents for Human SP-D Quantikine ELISA Kit
Product Specific Notices for Human SP-D Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.