Human TIGIT DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY7898-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
21.9-1400 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human TIGIT DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human TIGIT DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human TIGHT. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human TIGIT DuoSet ELISA
Human TIGIT DuoSet ELISA Standard Curve
Kit Contents for Human TIGIT DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates):(Catalog #DY008B) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS:(Catalog #DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer:(Catalog #WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: ELISA TMB Substrate (R&D Systems,
Catalog #DY999B)
Stop Solution:2N H2SO4(Catalog #DY994)
Microplates: R&D Systems (Catalog #DY990)
Plate Sealers:ELISA Plate Sealers (Catalog #DY992)
*For the Reagent Diluent and Blocking Buffer arerecommended for a specific DuoSet ELISA Development Kit,see the product datasheet.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TIGIT
Long Name
T Cell Immunoreceptor with Ig and ITIM Domains
Alternate Names
VSIG9, VSTM3, WUCAM
Gene Symbol
TIGIT
Additional TIGIT Products
Product Documents for Human TIGIT DuoSet ELISA
Product Specific Notices for Human TIGIT DuoSet ELISA
For research use only
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