Human ULBP-1 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY1380
Key Product Details
Assay Type
Assay Range
Sample Type
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human ULBP-1 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human ULBP-1 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human ULBP-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
Sample Volume Required
Detection Method
Conjugate
Specificity
Label
Scientific Data Images for Human ULBP-1 DuoSet ELISA
Human ULBP-1 ELISA Standard Curve
Kit Contents for Human ULBP-1 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Preparation and Storage
Shipping
Stability & Storage
Background: ULBP-1
MULT1 (mouse UL16-binding protein-like Transcript 1) is a 53 kDa, MHC Class I-like molecule that belongs to the mouse family of NKG2D ligands. It is a type I transmembrane glycoprotein that is synthesized as a 334 amino acid (aa) precursor. It contains a 25 aa signal sequence, a 186 aa extracellular region, a 19 aa transmembrane segment and a 104 aa cytoplasmic tail. The extracellular region contains an alpha-1 and alpha-2 like domain with two intrachain disulfide bonds.
Long Name
Alternate Names
Gene Symbol
Additional ULBP-1 Products
Product Documents for Human ULBP-1 DuoSet ELISA
Product Specific Notices for Human ULBP-1 DuoSet ELISA
For research use only