Human Uteroglobin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DUGB00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (50 µL), Urine (50 µL), Human Milk (50 µL)
Sensitivity
0.217 ng/mL
Assay Range
0.78-50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Product Summary for Human Uteroglobin Quantikine ELISA Kit
The Quantikine Human Uteroglobin Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Uteroglobin in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains E. coli-expressed recombinant human Uteroglobin and has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural human Uteroglobin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Uteroglobin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Uteroglobin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 5.2 | 15.8 | 31.7 | 5.0 | 14.7 | 28.8 |
| Standard Deviation | 0.21 | 0.49 | 1.16 | 0.28 | 0.92 | 2.09 |
| CV% | 4.0 | 3.1 | 3.7 | 5.6 | 6.3 | 7.3 |
Human Milk, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 5.1 | 15.4 | 31.3 | 5.2 | 15.4 | 29.9 |
| Standard Deviation | 0.29 | 0.34 | 1.04 | 0.31 | 0.72 | 2.25 |
| CV% | 5.7 | 2.2 | 3.3 | 6.0 | 4.7 | 7.5 |
Recovery for Human Uteroglobin Quantikine ELISA Kit
The recovery of Uteroglobin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 99-109 |
| EDTA Plasma (n=4) | 95 | 85-107 |
| Heparin Plasma (n=4) | 95 | 86-109 |
| Serum (n=4) | 95 | 85-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Uteroglobin were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Uteroglobin Quantikine ELISA Kit
Human Uteroglobin/SCGB1A1 ELISA Saliva/Urine/Human Milk Standard Curve
Human Uteroglobin/SCGB1A1 ELISA Cellculture Supernate/Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Uteroglobin/SCGB1A1
Alternate Names
Blastokinin, CC10, CC16, CCPBP, CCSP, Clara, PCB-BP, SCGB1A1, UGB
Gene Symbol
SCGB1A1
Additional Uteroglobin/SCGB1A1 Products
Product Documents for Human Uteroglobin Quantikine ELISA Kit
Product Specific Notices for Human Uteroglobin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.