Human VEGFR1/Flt-1 Quantikine ELISA Kit

Name: Human VEGFR1/Flt-1 Quantikine ELISA Kit
Brand: R&D Systems
SKU: DVR100B

R&D Systems, part of Bio-Techne | Catalog # DVR100B

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Product Datasheet / COA / SDS

New Version Available - DVR100C

Discontinued Product

DVR100B has been discontinued. An alternative/replacement product is available: DVR100C. View all VEGFR1/Flt-1 products.

Key Product Details

Assay Length:

4.5 hours

Human VEGFR1/Flt-1 Quantikine ELISA Kit Summary:

The Quantikine Human VEGF R1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human soluble VEGF R1 in cell culture supernates, serum, plasma, saliva, and follicular fluid. It contains Sf 21-expressed recombinant human VEGF R1/Fc Chimera and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human VEGF R1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring soluble VEGF R1.

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View Full Technical Details for Human VEGFR1/Flt-1 Quantikine ELISA Kit

Technical Data
Citations / Reviews

Product Specifications for Human VEGFR1/Flt-1 Quantikine ELISA Kit

Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human soluble VEGF R1
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Saliva, Follicular Fluid

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	96.6	596	1213	112	645	1279
Standard Deviation	2.5	22.8	39.3	11	44.9	70.5
CV%	2.6	3.8	3.2	9.8	7	5.5

Recovery

The recovery of VEGF R1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	100	86-113
EDTA Plasma (n=4)	99	89-105
Serum (n=4)	103	96-107

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of VEGF R1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human VEGFR1/Flt-1 Quantikine ELISA Kit

	Human VEGF R1/Flt-1 Standard Curve
	Human VEGF R1 Quantikine ELISA Kit Linearity Competitor Comparison
	Human VEGF R1 Quantikine ELISA Kit Sensitivity Competitor Comparison
	Human VEGF R1 Quantikine ELISA Kit Interference Competitor Comparison
	Human VEGF R1/Flt-1 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGFR1/Flt-1

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

Item	Value
Long Name	Vascular Endothelial Growth Factor Receptor 1
Entrez Gene IDs	2321 (Human); 14254 (Mouse)
Alternate Names	EC 2.7.10, EC 2.7.10.1, FLT, FLT1, FRT, Flt-1, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, VEGF R1, VEGFR-1, VEGFR1, Vascular permeability factor receptor, fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor), vascular endothelial growth factor receptor 1

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