Human VEGFR1/Flt-1 Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DVR100C
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Cell Lysates (10 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Saliva (100 µL)
Sensitivity
8.46 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Product Summary for Human VEGFR1/Flt-1 Quantikine ELISA Kit
The Quantikine® Human VEGF R1/Flt-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VEGF R1 in cell culture supernates, cell lysates, serum, plasma, and saliva. It contains NS0-expressed recombinant human VEGF R1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human VEGF R1 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human VEGF R1.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human VEGF R1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
| Sample Type | Mean (pg/mL | Range (pg/mL) |
| Serum (n=30) | 385 | 294-478 |
| EDTA Plasma (n=30) | 292 | 208-391 |
| Heparin Plasma (n=30) | 279 | 216-377 |
| Saliva (n=10) | 944 | 346-1827 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Saliva, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 207 | 610 | 1207 | 207 | 616 | 1205 |
| Standard Deviation | 5.16 | 14.9 | 35.6 | 13.9 | 38.9 | 74.0 |
| CV% | 2.5 | 2.4 | 2.9 | 6.7 | 6.3 | 6.1 |
Recovery for Human VEGFR1/Flt-1 Quantikine ELISA Kit
The recovery of human VEGF R1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 99 | 87-110 |
| Cell Lysis Buffer (n=4) | 94 | 86-108 |
| EDTA Plasma (n=4) | 92 | 84-98 |
| Heparin Plasma (n=4) | 89 | 81-96 |
| Saliva (n=4) | 109 | 96-124 |
| Serum (n=4) | 104 | 93-114 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human VEGF R1 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human VEGFR1/Flt-1 Quantikine ELISA Kit
Human VEGF R1/Flt-1 Standard Curve
Human VEGF R1 Quantikine ELISA Kit Linearity Competitor Comparison
Human VEGF R1 Quantikine ELISA Kit Sensitivity Competitor Comparison
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: VEGFR1/Flt-1
VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.
Long Name
Vascular Endothelial Growth Factor Receptor 1
Alternate Names
Flt-1, FLT1, FRT, VEGF R1, VEGFR-1
Gene Symbol
FLT1
Additional VEGFR1/Flt-1 Products
Product Documents for Human VEGFR1/Flt-1 Quantikine ELISA Kit
Product Specific Notices for Human VEGFR1/Flt-1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.