Human XCL1/Lymphotactin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DXCL10
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
Sensitivity
0.023 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human XCL1/Lymphotactin Quantikine ELISA Kit
The Quantikine Human XCL1/Lymphotactin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Lymphotactin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Lymphotactin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Lymphotactin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Lymphotactin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Lymphotactin. This assay also recognizes human XCL2.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.920 | 2.83 | 5.57 | 1.01 | 3.08 | 6.29 |
| Standard Deviation | 0.024 | 0.078 | 0.116 | 0.095 | 0.278 | 0.494 |
| CV% | 2.6 | 2.8 | 2.1 | 9.4 | 9.0 | 7.9 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.12 | 3.27 | 6.66 | 1.14 | 3.32 | 6.69 |
| Standard Deviation | 0.031 | 0.085 | 0.184 | 0.084 | 0.199 | 0.374 |
| CV% | 2.8 | 2.6 | 2.8 | 7.4 | 6.0 | 5.6 |
Recovery for Human XCL1/Lymphotactin Quantikine ELISA Kit
The recovery of human Lymphotactin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 109 | 99-116 |
| EDTA Plasma (n=4) | 95 | 86-100 |
| Heparin Plasma (n=4) | 93 | 87-101 |
| Serum (n=4) | 98 | 89-107 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human Lymphotactin were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human XCL1/Lymphotactin Quantikine ELISA Kit
Human XCL1/Lymphotactin ELISA Cell Culture Supernate Standard Curve
Human XCL1/Lymphotactin ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: XCL1/Lymphotactin
Long Name
Chemokine [C Motif] Ligand 1
Alternate Names
ATAC, Lymphotactin, SCM-1 alpha
Gene Symbol
XCL1
Additional XCL1/Lymphotactin Products
Product Documents for Human XCL1/Lymphotactin Quantikine ELISA Kit
Product Specific Notices for Human XCL1/Lymphotactin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.