Hyaluronan ELISA Kit - Quantikine Best Seller
R&D Systems, part of Bio-Techne | Catalog # DHYAL0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity
0.2 ng/mL
Assay Range
0.625-40 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Hyaluronan Quantikine ELISA Kit
The Quantikine Hyaluronan Immunoassay is a 4.5 hour solid-phase ELISA designed to measure at least 35 kDa Hyaluronan in human, mouse, rat, canine, and porcine cell culture supernates, tissue homogenates, serum, and plasma samples. It contains Streptococcus pyogenes-derived Hyaluronan and NS0-expressed human Aggrecan. This immunoassay has been shown to accurately quantitate Hyaluronan.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Multi-Species
Specificity
Measures greater than or equal to 35 kDa Hyaluronan in human, mouse, rat, canine, and porcine samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 1.53 | 4.81 | 14.3 | 1.68 | 4.68 | 14.98 |
| Standard Deviation | 0.11 | 0.26 | 0.52 | 0.08 | 0.23 | 0.72 |
| CV% | 7.2 | 5.4 | 3.6 | 4.8 | 4.9 | 4.8 |
Recovery for Hyaluronan Quantikine ELISA Kit
The recovery of Hyaluronan spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 104 | 96-116 |
| EDTA Plasma (n=10) | 97 | 83-106 |
| Heparin Plasma (n=10) | 99 | 80-124 |
| Serum (n=10) | 98 | 81-119 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of Hyaluronan were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Hyaluronan Quantikine ELISA Kit
Human, Mouse, Rat, Porcine, Canine Hyaluronan ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Hyaluronan
Additional Hyaluronan Products
Product Documents for Hyaluronan Quantikine ELISA Kit
Product Specific Notices for Hyaluronan Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.