Human ICAM-1/CD54 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82420

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, heparin, citrate), amniotic fluid, spontaneous urine, bile, other body fluids (10 uL)

Sensitivity

2.2 ng/mL

Assay Range

6.25 - 100 ng/mL

View all ICAM-1/CD54 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This antibody is reactive to the soluble protein. There was no cross reactivity detected with TNF-R (60 kDa), TNF-R (80 kDa), IL-8/NAP-1, TNF alpha, TNF beta, IFN gamma, IFN alpha 2C, IFN omega, IL-6, IL-2R, ELAM-1 and L-selectin.

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.1%

Inter-Assay Precision (Precision between assays) CV% < 7.7%

Recovery for Human ICAM-1/CD54 ELISA Kit (Colorimetric)

Recovery

The recovery ranged from 82% to 109% with an overall mean recovery of 99%.

Scientific Data Images for Human ICAM-1/CD54 ELISA Kit (Colorimetric)

ELISA: Human ICAM-1/CD54 ELISA Kit (Colorimetric) [NBP1-82420]

ELISA: Human ICAM-1/CD54 ELISA Kit (Colorimetric) [NBP1-82420]

ELISA: ICAM-1/CD54 ELISA Kit (Colorimetric) [NBP1-82420] - Representative standard curve for human ICAM-1 ELISA. Human ICAM-1 was diluted in serial 2-fold steps in Sample Diluent. Do not use this standard curve to derive test results. A standard curve must be runfor each group of microwell strips assayed.

Kit Contents for Human ICAM-1/CD54 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch with a Microwell Plate coated with monoclonal antibody to human ICAM-1/CD54
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Control high
  • Control low
  • HRP-Conjugate anti-human ICAM-1/CD54 monoclonal antibody
  • Human ICAM-1/CD54 Standard
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1) is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1), an alpha-betacomplex that is a member of the leukocyte integrin family of cell-cell and cell-matrix receptors. ICAM-1 is a single-chain glycoprotein with a polypeptide core of 55 kD that can be expressed on non-hematopoietic cells of many lineages such as vascular endothelial cells, thymic epithelial cells, other epithelial cells and fibroblasts and on hematopoietic cells such as tissue macrophages, mitogen-stimulated T-lymphoblasts, germinal center B cells and dendritic cells in tonsils, lymph nodes and Peyer's patches. ICAM-1 is inducible on fibroblasts and endothelial cells by inflammatory mediators such as IL-1, TNF and IFN-gamma within few hours and is correlated to the infiltration of lymphocytes into inflammatory lesions. ICAM-1 seems to be the initial marker of inflammatory reactions and is expressed prior to, and to a greater extent than is HLA-DR. sICAM-1 serum levels were found elevated in acute renal graft rejection, insulin-dependent diabetis mellitus, anterior uveitis and in allergic inflammation.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for Human ICAM-1/CD54 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human ICAM-1/CD54 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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