Monkey ICAM-1/CD54 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92712

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (baboon, rhesus serum) (10 uL)

Sensitivity

1.1 U/mL

Assay Range

6.3 - 100 U/mL

View all ICAM-1/CD54 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Monkey

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a monkey ICAM-1 positive serum. There was no cross reactivity detected.

Description

The monkey sICAM-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of monkey sICAM-1

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.0%

Inter-Assay Precision (Precision between assays) CV% < 10%

Recovery for Monkey ICAM-1/CD54 ELISA Kit (Colorimetric)

Recovery

The overall mean recovery was 95%. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Monkey ICAM-1/CD54 ELISA Kit (Colorimetric)

Sandwich ELISA: Monkey ICAM-1/CD54 ELISA Kit (Colorimetric) [NBP1-92712] - Representative standard curve for monkey ICAM-1 ELISA. Monkey ICAM-1 was diluted in serial 2-fold steps in Sample Diluent. Do not use this standard curve to derive test results. A standard curve must be run for each group of microwell strips assayed.

Kit Contents for Monkey ICAM-1/CD54 ELISA Kit (Colorimetric)

  • Adhesive Plate Covers
  • Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to monkey sICAM-1 and HRP-Conjugate (anti-monkey sICAM-1 monoclonal (murine) antibody, Sample Diluent lyophilized
  • Aluminium pouch(es) with a monkey sICAM-1 Standard curve (coloured)
  • Sample Diluent (Use when an external predilution of the samples is needed)
  • Stop Solution (1M Phosphoric acid)
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: ICAM-1/CD54

ntercellular Adhesion Molecule-1 (ICAM-1) is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1), an alpha-beta-complex that is a member of the leukocyte integrin family of cell-cell and cell-matrix receptors. This family consists of the leukocyte adhesion glycoproteins LFA-1 which mediates lymphocyte adhesion, Mac-1 which mediates granulocyte adhesion and p150,95. ICAM-1 is a single-chain glycoprotein with a polypeptide core that can be expressed on non-hematopoietic cells of many lineages such as vascular endothelial cells, thymic epithelial cells, other epithelial cells and fibroblasts and on hematopoietic cells such as tissue macrophages, mitogen-stimulated T-lymphoblasts, germinal center B-cells and dendritic cells in tonsils, lymph nodes and Peyer's patches. ICAM-1 is inducible on fibroblasts and endothelial cells by inflammatory mediators such as IL-1, TNF and IFN-gamma within few hours and is correlated to the infiltration of lymphocytes into inflammatory lesions. ICAM-1 seems to be the initial marker of inflammatory reactions and is expressed prior to, and to a greater extent than is HLA-DR. The role of ICAM-1 as a disease marker has been demonstrated for a number of different indications and pathological situations.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for Monkey ICAM-1/CD54 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Monkey ICAM-1/CD54 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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