Human IgE ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60059

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 - 100 uL)

Sensitivity

3.640 ng/mL

Assay Range

62.5 ng/mL - 4000 ng/mL

View all IgE ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human IgE. No significant cross-reactivity or interference between human IgE and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Scientific Data Images for Human IgE ELISA Kit (Colorimetric)

ELISA: Human IgE ELISA Kit (Colorimetric) [NBP2-60059]
Human IgE ELISA Kit (Colorimetric)

Human IgE ELISA Kit (Colorimetric) [NBP2-60059] -

Human IgE ELISA Kit (Colorimetric) [NBP2-60059] - Standard Curve Reference

Kit Contents for Human IgE ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate (12 x 8 coated Microwells)
  • Biotin-antibody (100 x concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin Diluent
  • Instruction manual
  • RP-avidin (100 x concentrate)
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IgE

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IgE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IgE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IgE bound in the initial step. The color development is stopped and the intensity of the color is measured.

Long Name

Immunoglobulin E

Alternate Names

IgE, immunoglobulin heavy constant epsilon

Gene Symbol

IGHE

Additional IgE Products

Product Documents for Human IgE ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IgE ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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