Mouse IGFBP-6 ELISA Kit (Colorimetric)

Name: Mouse IGFBP-6 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-80248

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80248

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 ul)

Sensitivity

37.5 pg/mL

Assay Range

62.5-4000 pg/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse IGFBP-6 in samples. No significant cross-reactivity or interference between Mouse IGFBP-6 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.46%

Inter-Assay Precision (Precision between assays) CV% < 4.57%

Recovery for Mouse IGFBP-6 ELISA Kit (Colorimetric)

Recovery

90-104%

Linearity

Scientific Data Images for Mouse IGFBP-6 ELISA Kit (Colorimetric)

ELISA: Mouse IGFBP-6 ELISA Kit (Colorimetric) [NBP2-80248] -

ELISA: Mouse IGFBP-6 ELISA Kit (Colorimetric) [NBP2-80248] - Standard Curve Reference

Kit Contents for Mouse IGFBP-6 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100X)
Concentrated HRP Conjugate (100X)
Concentrated Wash Buffer (25X)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IGFBP-6

Data show that an increase in JNK activation in the presence of NFkappaB inhibition significantly increased the expression of IGFBP6. These studies provide evidence that over expression of IGFBP-6 suppresses human and murine osteoblast differentiation, that IGFBP-6 and LMP-1 physically interact, and supports the conclusion that this interaction may be functionally relevant. IGFBP-6 is the effector of tumor suppressor activity of SEMA3B. Observational study of gene-disease association. (HuGE Navigator) IGFBP-6 is translocated to the nucleus with functional consequences, and different members of the IGFBP family have specific nuclear import mechanisms. Toxic effect of TCDD on osteogenesis through altering IGFBP6 gene expression in osteoblasts is reported. p38 MAPK is involved in IGFBP-6-induced IGF-independent rhabdomyosarcoma cell migration. determination of blood levels in adult patients with severe liver disease before and after orthotopic liver transplantation. Inhibition of human osteoblast marker gene expression by retinoids is mediated in part by insulin-like growth factor binding protein-6. A close correlation exists between residues of the carboxyl-terminal domain of IGFBP6 undergoing conformational change in 15N NMR spectroscopy studies with those that disappeared or broadened upon insulin-like growth factor (IGF-II) binding. C-domain of IGFBP-6 consists of a thyroglobulin type 1 fold comprising an alpha-helix followed by a loop, a three-stranded antiparallel beta-sheet incorporating a second loop, and a disulfide-bonded flexible third loop. The expression of certain IGFBP is significantly altered in renal cell carcinoma. CCI-779 acts additively with IGFBP-6 to reduce rhabdomyosarcoma growth both in vitro and in vivo. may function as an antiproliferative molecule suppressing mitogenic effects of insulin-like growth factors on Malassez cells. The IGFBP-6 protein is synthesized as propeptides with a hydrophobic leader sequence, removal of which yields a mature protein composed of 3 recognizable domains of similar size. transgenic human IGFBP-6 mice may be considered a new tool for studies of the involvement of the brain IGF system in metabolism control and obesity.

Long Name

Insulin-like Growth Factor Binding Protein 6

Alternate Names

IGFBP6

Gene Symbol