Porcine IgG ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60065

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell culture supernates and tissue homogenates (50 - 100 uL)

Sensitivity

0.467 ug/mL

Assay Range

0.586 ug/mL - 150 ug/mL

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Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Porcine

Specificity

This assay has high sensitivity and excellent specificity for detection of pig IgG. No significant cross-reactivity or interference between pig IgG and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Scientific Data Images for Porcine IgG ELISA Kit (Colorimetric)

ELISA: Porcine IgG ELISA Kit (Colorimetric) [NBP2-60065]

ELISA: Porcine IgG ELISA Kit (Colorimetric) [NBP2-60065]

ELISA: Porcine IgG ELISA Kit (Colorimetric) [NBP2-60065] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Porcine IgG ELISA Kit (Colorimetric)

Porcine IgG ELISA Kit (Colorimetric) [NBP2-60065] -

Porcine IgG ELISA Kit (Colorimetric) [NBP2-60065] - Standard Curve Reference

Kit Contents for Porcine IgG ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate
  • Instruction manual
  • Sample Diluent
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IgG

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with pig IgG. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for pig IgG. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of pig IgG in samples. The color development is stopped and the intensity of the color is measured.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for Porcine IgG ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Porcine IgG ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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