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Human IgG4 ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00407

Novus Biologicals, part of Bio-Techne
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NBP3-00407

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.94 ng/mL

Assay Range

1.56 - 100 ng/mL

Product Specifications

Assay Type

Competitive-ELISA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human IgG4 in samples. No significant cross-reactivity or interference between Human IgG4 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 11.44%

Inter-Assay Precision (Precision between assays) 8.85%

Recovery for Human IgG4 ELISA Kit (Chemiluminescence)

Recovery

89 - 111%

Linearity

Human IgG4 ELISA Kit (Chemiluminescence)

Scientific Data Images for Human IgG4 ELISA Kit (Chemiluminescence)

Human IgG4 ELISA Kit (Chemiluminescence)

ELISA: Human IgG4 ELISA Kit (Chemiluminescence) [NBP3-00407] -

ELISA: Human IgG4 ELISA Kit (Chemiluminescence) [NBP3-00407] - Standard Curve Reference

Kit Contents for Human IgG4 ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IgG4

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G4

Alternate Names

Immunoglobulin G, Immunoglobulin G4, ImmunoglobulinG

Additional IgG4 Products

Product Documents for Human IgG4 ELISA Kit (Chemiluminescence)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IgG4 ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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