Mouse IL-12/IL-35 p35 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06743

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL

View all IL-12/IL-35 p35 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse IL-12 p70/IL-12A in samples. No significant cross-reactivity or interference between Mouse IL-12 p70/IL-12A and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 5.05

Inter-Assay Precision (Precision between assays) 5.35

Recovery for Mouse IL-12/IL-35 p35 ELISA Kit (Colorimetric)

Recovery

85 - 108 %

Linearity

Mouse IL-12/IL-35 p35 ELISA Kit (Colorimetric)

Scientific Data Images for Mouse IL-12/IL-35 p35 ELISA Kit (Colorimetric)

Mouse IL-12/IL-35 p35 ELISA Kit (Colorimetric)

ELISA: Mouse IL-12/IL-35 p35 ELISA Kit (Colorimetric) [NBP3-06743] -

ELISA: Mouse IL-12/IL-35 p35 ELISA Kit (Colorimetric) [NBP3-06743] - Standard Curve Reference

Kit Contents for Mouse IL-12/IL-35 p35 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-12/IL-35 p35

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

Long Name

Interleukin 12 p35

Alternate Names

IL-12A, IL35p35

Gene Symbol

IL12A

Additional IL-12/IL-35 p35 Products

Product Documents for Mouse IL-12/IL-35 p35 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse IL-12/IL-35 p35 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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