Porcine IL-12/IL-35 p35 ELISA Kit (Colorimetric)

Name: Porcine IL-12/IL-35 p35 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-42647

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-42647

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Product Specifications
Precision
Recovery
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids (100 uL)

Sensitivity

6.3 pg/mL

Assay Range

15.6 - 1000 pg/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Porcine

Description

Assay time: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10

Inter-Assay Precision (Precision between assays) %CV < 12

Recovery for Porcine IL-12/IL-35 p35 ELISA Kit (Colorimetric)

Recovery

91 - 104%

Kit Contents for Porcine IL-12/IL-35 p35 ELISA Kit (Colorimetric)

Assay Diluent A
Assay Diluent B
Detection Reagent A
Detection Reagent B
Instruction manual
Plate sealer for 96 wells
Pre-coated ready to use 96-well strip plate
Standard
Standard Diluent
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-12/IL-35 p35

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

Long Name

Interleukin 12 p35

Alternate Names

IL-12A, IL35p35

Gene Symbol

IL12A

Additional IL-12/IL-35 p35 Products

Product Documents for Porcine IL-12/IL-35 p35 ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Porcine IL-12/IL-35 p35 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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