Human IL-17/IL-17A ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82423

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (citrate, heparin), buffered solution (50 ul)

Sensitivity

0.5 pg/mL

Assay Range

1.6 - 100 pg/mL

View all IL-17/IL-17A ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no crossreactivity detected.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 7.1%

Inter-Assay Precision (Precision between assays) CV% < 9.1%

Recovery for Human IL-17/IL-17A ELISA Kit (Colorimetric)

Recovery

The overall mean recovery in serum samples was 107.0%, in plasma (heparin) samples 50.8%, in plasma (citrate) 75.5% and in cell culture supernatant samples 113.6%.

Scientific Data Images for Human IL-17/IL-17A ELISA Kit (Colorimetric)

ELISA: Human IL-17/IL-17A ELISA Kit (Colorimetric) [NBP1-82423]

ELISA: Human IL-17/IL-17A ELISA Kit (Colorimetric) [NBP1-82423]

ELISA: IL-17/IL-17A ELISA Kit [NBP1-82423] - Standard curve

Kit Contents for Human IL-17/IL-17A ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-17A
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human IL-17A monoclonal antibody
  • Control high, lyophilized
  • Control low, lyophilized
  • Human IL-17A Standard lyophilized, 200 pg/mL upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-17/IL-17A

The T help 1 (Th1) and Th2 cell classification has provided the framework for understanding CD4(+) T cell biology and the interplay between innate and adaptive immunity. Studies have defined an novel arm of the CD4(+) T cell effector response, the Th17 lineage. IL-17A, a homodimeric cytokine of about 32 kDa, is largely produced by activated memory T lymphocytes, but stimulates innate immunity and host defense. The closest associate to IL-17A is IL-17F, which is encoded in a syntenic fashion and shares around 50% sequence homology. IL-17A and IL-17F both mobilize neutrophils partly through granulopoeisis and CXC chemokine induction. Increased levels of IL-17 have been associated with several conditions, including airway inflammation, rheumatoid arthritis, intraperitoneal abscesses and adhesions, inflammatory bowel disease, allograft rejection, psoriasis, cancer and multiple sclerosis.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Gene Symbol

IL17A

Additional IL-17/IL-17A Products

Product Documents for Human IL-17/IL-17A ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IL-17/IL-17A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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