IL-17/IL-17A ELISA Development Kit

Name: IL-17/IL-17A ELISA Development Kit
Brand: Novus Biologicals
SKU: DDXK-E-IL17A

Novus Biologicals, part of Bio-Techne | Catalog # DDXK-E-IL17A

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Product Specifications
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Type

Sandwich ELISA

Assay Range

0.31ng/ml - 20ng/mL

Sample Type

Human cell culture supernatants, plasma, serum, and various biological fluids (100 uL)

Sensitivity

1 ng/ml

Reactivity

Human

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Product Specifications

Sample Volume Required

100 uL

Conjugate

Unconjugated

Application Notes

The human IL17 ELISA kit is for quantitative determination of IL17 concentrations in human cell culture supernatants and biological fluids (plasma, serum). This kit is for research purpose only.

Scientific Data Images for IL-17/IL-17A ELISA Development Kit

ELISA: IL-17/IL-17A ELISA Development Kit [DDXK-E-IL17A]

ELISA: Human IL-17/IL-17A ELISA Kit [DDXK-E-IL17A] - Example of standard curve

Kit Contents for IL-17/IL-17A ELISA Development Kit

Capture Antibody: 0.5 mg/mL of mouse anti-IL17 monoclonal antibody (clone 408H6.01). Dilute in 50mM carbonate buffer pH 9.6 to a working concentration of 2.5 ug /mL
Detection Antibody: 0.5mg/mL of HRPO-conjugated anti-IL17 mouse monoclonal antibody (clone 405B4.01). Dilute to a working concentration of 3ug/mL in PBS-BSA 1%- Tween 0.05%.
Standard: Each vial contains 1ug/mL of recombinant IL17 produced, purified and concentrated from eukaryotic cells. A 7-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 20 ng /mL is recommended

Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-17/IL-17A

IL17 (cytotoxic T lymphocyte associated antigen 8) is a CD4+T cell-derived cytokine that stimulates stromal cells and macrophages to secrete proinflammatory cytokines. IL17 regulates the activities of NF-kappa B and mitogen-activated protein kinases. hIL17 stimulates epithelial, endothelial, and fibroblastic cells to secrete cytokines such as IL6, IL8, and G-CSF and PGE2. IL17 promoted the maturation of DC progenitors, as evidenced by increased cell surface expression of costimulatory molecules and MHC class II Ag, and allostimulatory capacity. IL17 had a lesser effect on the phenotype and function of more fully differentiated myeloid DC. These findings suggest a role for IL17 in allogeneic T cell proliferation that may be mediated in part via a maturation-inducing effect on DC. IL17 appears to be a novel target for therapeutic intervention in allograft rejection. High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. (Fossiez F. et al, 1996 ; J.Exp.Med, 183:2593-2603 ; Fossiez F. et al, 1998 ; Int. Rev. Immunol., 16:541-551).

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Gene Symbol

IL17A

Additional IL-17/IL-17A Products

Product Documents for IL-17/IL-17A ELISA Development Kit

Product Datasheets

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Protocols & Manuals

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COA

Product Specific Notices for IL-17/IL-17A ELISA Development Kit

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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