Human IL-17E/IL-25 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68035

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL

View all IL-17E/IL-25 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human IL-25 in samples. No significant cross-reactivity or interference between Human IL-25 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.66%

Inter-Assay Precision (Precision between assays) CV% < 5.15%

Recovery for Human IL-17E/IL-25 ELISA Kit (Colorimetric)

Recovery

84-107%

Linearity

Human IL-17E/IL-25 ELISA Kit (Colorimetric)

Scientific Data Images for Human IL-17E/IL-25 ELISA Kit (Colorimetric)

Human IL-17E/IL-25 ELISA Kit (Colorimetric)

ELISA: Human IL-17E/IL-25 ELISA Kit (Colorimetric) [NBP2-68035] -

ELISA: Human IL-17E/IL-25 ELISA Kit (Colorimetric) [NBP2-68035] - Standard Curve Reference

Kit Contents for Human IL-17E/IL-25 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-17E/IL-25

Recently, a number of cytokines belonging to the interleukin (IL)-17 family have been identified. These are termed as IL-17B, IL-17C (1) and IL-17E (2). IL-17 is a potent proinflammatory cytokine that plays roles in a number of diseases including rheumatoid arthritis (3), multiple sclerosis (4), and promotion of tumor growth (5). IL-17B, C, and E like IL-17 are able to induce proinflammatory responses. However, they do not bind to the IL-17 receptor suggesting that additional IL-17R related receptor might exist. Receptor for IL-17B and IL-17E has been independently isolated by Shi, et al (6) and Lee, et al. (2) and has been designated as EV127 (in mouse) and IL-17Rh1 (in human), respectively. IL-17E induces activation of NF-kB pathway and like IL-17 also induces production of IL-8.

Long Name

Interleukin 17E

Alternate Names

IL-25, IL17E, IL25

Gene Symbol

IL25

Additional IL-17E/IL-25 Products

Product Documents for Human IL-17E/IL-25 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IL-17E/IL-25 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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