Monkey IL-2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92709

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

cell culture supernatant, serum, plasma (EDTA, citrate, heparin) (50 uL)

Sensitivity

9.1 U/mL

Assay Range

18.8-1,200 U/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Monkey

Specificity

The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a monkey IL-2 positive serum. There was no cross reactivity or interference detected.

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 7.0%

Inter-Assay Precision (Precision between assays) CV% < 5.0%

Scientific Data Images for Monkey IL-2 ELISA Kit (Colorimetric)

ELISA: Monkey IL-2 ELISA Kit (Colorimetric) [NBP1-92709]

Kit Contents for Monkey IL-2 ELISA Kit (Colorimetric)

  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to monkey IL-2

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-2

IL-2 plays a central role in the activation and proliferation of lymphocytes that have been primed by antigens. IL-2 plays a pivotal role in for the expansion of most T-cells, natural killer cells and B-cells during certain phases of their response. IL-2 is a 15 kDa glycoprotein encoded by a single gene located in the q26-28 region of human chromosome 4. The cDNA deduced polypeptide consists of 153 amino acids. Apart from its most important role to mediate antigen-specific T-lymphocyte proliferation, IL-2 modulates the expression of interferon gamma and major histocompatibility antigens, stimulates proliferation and differentiation of activated B-cells, augments natural killer cell activity and inhibits granulocyte-macrophage colony formation. IL-2 displays antitumoral effects, thus being used in cancer therapy. Monitoring of IL-2 levels in serum provides more detailed insights in several pathological situations such as cancer, infectious diseases, transplant rejection, multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus and type I diabetes.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Gene Symbol

IL2

Additional IL-2 Products

Product Documents for Monkey IL-2 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Monkey IL-2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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