Human IL-21 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84816

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate), buffered solution (50 uL)

Sensitivity

20.0 pg/mL

Assay Range

78 - 5,000 pg/mL

View all IL-21 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The assay detects both natural and recombinant human IL-21. The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no crossreactivity detected.

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.8%

Inter-Assay Precision (Precision between assays) CV% < 7.7%

Recovery for Human IL-21 ELISA Kit (Colorimetric)

Recovery

The mean recovery for sera was 71%, for plasma (EDTA) 42%, for plasma (citrate) 58% and for cell culture supernatant 95%.

Scientific Data Images for Human IL-21 ELISA Kit (Colorimetric)

ELISA: Human IL-21 ELISA Kit (Colorimetric) [NBP1-84816]

ELISA: Human IL-21 ELISA Kit (Colorimetric) [NBP1-84816]

ELISA: IL-21 ELISA Kit [NBP1-84816] - Standard curve

Kit Contents for Human IL-21 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-21
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human IL-21 monoclonal antibody
  • Human IL-21 Standard lyophilized, 10 ng/mL upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-21

The human IL-21 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-21. Interleukin-21 is a member of the type-I cytokine family. Structurally, IL-21 shows homology to IL-2, IL-4, and IL-15 proteins. IL-21 shares the common gamma-chain with these three cytokines, but, in addition, binds to a unique IL-21R alpha chain, and activates the JAK/STAT pathway. IL-21 has pleiotropic actions, from augmenting the proliferation of T cells and driving the differentiation of B cells into memory cells and terminally differentiated plasma cells to augmenting the activity of natural killer cells. IL-21 is mainly produced by activated T-cells, but targets a broad range of lymphoid and myeloid cells of the immune system and therefore is able to regulate innate and acquired immune responses. IL-21 has pathologic function in immune-inflammatory diseases. Data suggest the existence of a positive autocrine loop that could help to amplify and stabilize IL-21-driven, T cell-mediated responses. Moreover, it has antitumour activity and may have a role in the development of autoimmunity. IL-21 may have therapeutic potentials as an antitumor agent in the clinic.

Long Name

Interleukin 21

Alternate Names

CVID11, IL21, Za11

Gene Symbol

IL21

Additional IL-21 Products

Product Documents for Human IL-21 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IL-21 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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