Mouse IL-23 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92664

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (citrate), buffered solution (50 uL)

Sensitivity

16.0 pg/mL

Assay Range

31.3 - 2,000 pg/mL

View all IL-23 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

The assay detects both natural and recombinant mouse IL-23. The interference of GM-CSF, IFN gamma, IL-1 alpha, IL-2, IL-4, IL-5, IL- 6, IL-10, IL-12p70, IL-13, IL-17, IL-18, IL-22, TNF alpha, MCP-1, MCP-3, MIP-1 alpha, MIP-1 beta, RANTES, IP-10 was evaluated by spiking these proteins at physiologically relevant concentrations into IL-23 positive serum. There was no crossreactivity detected.

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 8.30%

Inter-Assay Precision (Precision between assays) CV% < 2.50%

Recovery for Mouse IL-23 ELISA Kit (Colorimetric)

Recovery

The unspiked serum was used as blank in these experiments. The recovery ranged from 41% to 53% with an overall mean recovery of 49%

Scientific Data Images for Mouse IL-23 ELISA Kit (Colorimetric)

Mouse IL-23 R ELISA Kit [NBP1-92664] - Data for Mouse IL-23 Platinum ELISA

Kit Contents for Mouse IL-23 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-23
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-mouse IL-23 monoclonal antibody
  • Mouse IL-23 Standard lyophilized, 4000 pg/ml upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-23

IL-23 (Interleukin-23) is a heterodimeric cytokine consisting of two subunits, p19 and p40. IL-23 plays an important role in the inflammatory response against infection. It is a key element in the pathogenesis of inflammatory bowel disease, which comprises Crohn's disease and ulcerative colitis. It can also have implications in the development of cancerous tumors. IL-23 promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T cell infiltration. In conjunction with IL-6 and TGF-b1, IL-23 stimulates naive CD4+ T cells to differentiate into a novel subset of cells called Th17 cells, which are distinct from the classical Th1 and Th2 cells. Th17 cells produce IL-17, a proinflammatory cytokine that enhances T cell priming and stimulates the production of proinflammatory molecules such as IL-1, IL-6, TNF-a, NOS-2, and chemokines resulting in inflammation (McGovern D et al, 2007).

Long Name

Interleukin 23

Alternate Names

IL-23 p19/IL-12 p40, IL-23p19/IL-12p40, IL23, SGRF

Gene Symbol

IL23A

Additional IL-23 Products

Product Documents for Mouse IL-23 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse IL-23 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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