Human IL-29/IFN-lambda 1 ELISA Kit (Colorimetric)

Name: Human IL-29/IFN-lambda 1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP1-84819

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84819

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Product Specifications
Precision
Recovery
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution (50 uL)

Sensitivity

2.0 pg/mL

Assay Range

15.6 - 1,000 pg/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The assay detects both natural and recombinant human IL-29/IFNlambda 1. The cross-reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-29/IFNlambda 1 positive sample. There was no cross-reactivity or interference detected.

Description

The human IL-29 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-29

Precision

Intra-Assay Precision (Precision within an assay) CV% < 7.6%

Inter-Assay Precision (Precision between assays) CV% < 7.1%

Recovery for Human IL-29/IFN-lambda 1 ELISA Kit (Colorimetric)

Recovery

Please See Protocol for Specific Spike Recovery Details

Scientific Data Images for Human IL-29/IFN-lambda 1 ELISA Kit (Colorimetric)

ELISA: Human IL-29/IFN-lambda 1 ELISA Kit (Colorimetric) [NBP1-84819]

ELISA: IL-29/IFN-lambda 1 ELISA Kit [NBP1-84819] - Standard curve

Kit Contents for Human IL-29/IFN-lambda 1 ELISA Kit (Colorimetric)

Adhesive Films
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-29
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Biotin-Conjugate anti-human IL-29 monoclonal antibody
Human IL-29 Standard lyophilized, 2 ng/ml upon reconstitution
Sample Diluent
Stop Solution (1M Phosphoric acid)
Streptavidin-HRP
Substrate Solution (tetramethyl-benzidine)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-29/IFN-lambda 1

IL-29 is a protein of the helical cytokine family and is a type III interferon sharing many functions with the type I family of interferons. The IL-29 gene is found on chromosome 19 in humans. It is also known as IFN-lambda1 and is highly similar in amino acid sequence to the IL-28, the other type III interferon. IL-29 does not bind the IFN-alpha/beta receptor, but instead signals through a receptor composed of the IL-28R1 and IL-10R2 subunits. Initial characterizations of the receptor demonstrated that it has a ubiquitous tissue distribution and is expressed on most non-hematopoietic cells. Generation of native IL-29 is achieved by monocytes and dendritic cells in response to viral infection and stimulation with toll-like receptor ligands. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses. IL 29 has significant antiviral activity and immunoregulatory properties and appears to inhibit T helper-2 (Th2) responses regarding inhibition of IL-13 production, compared with IL-4 or IL-5. The antiviral activities of IL-29 include the upregulation of MHC Class I expression on the cell surface and the expression of PKR. The ligand/receptor complex seems to signal through the Jak-STAT pathway.

Long Name

Interleukin 29

Alternate Names

IFN-lambda 1, IL29

Gene Symbol

IFNL1

Additional IL-29/IFN-lambda 1 Products

Product Documents for Human IL-29/IFN-lambda 1 ELISA Kit (Colorimetric)

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download Manual - Human IL-29/IFN-lambda 1 ELISA Kit (Colorimetric)

COA

SDS

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Product Specific Notices for Human IL-29/IFN-lambda 1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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