Human IL-33 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21492

Novus Biologicals, part of Bio-Techne
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NBP3-21492
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 ul)

Sensitivity

9.38 pg/mL

Assay Range

15.63-1000 pg/mL

View all IL-33 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human IL-33 in samples. No significant cross-reactivity or interference between Human IL-33 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV < 10%

Inter-Assay Precision (Precision between assays) CV < 10%

Recovery for Human IL-33 ELISA Kit (Colorimetric)

Recovery

97-102%

Scientific Data Images for Human IL-33 ELISA Kit (Colorimetric)

Human IL-33 ELISA Kit (Colorimetric)

Samples were spiked with high concentrations of Human IL-33 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Human IL-33 ELISA Kit (Colorimetric)

ELISA: Human IL-33 ELISA Kit (Colorimetric) [NBP3-21492] -

ELISA: Human IL-33 ELISA Kit (Colorimetric) [NBP3-21492] - Standard Curve Reference

Kit Contents for Human IL-33 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-33

Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.

Long Name

Interleukin 33

Alternate Names

C9orf26, DVS27, IL33, NF-HEV

Gene Symbol

IL33

Additional IL-33 Products

Product Documents for Human IL-33 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IL-33 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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