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IL-38/IL-1F10 Antibody Pair

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-78862

Novus Biologicals, part of Bio-Techne
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NBP2-78862

Key Product Details

Assay Type

Sandwich ELISA

Assay Range

0.031 - 2 ng/ml

Sample Type

Cell Culture Supernatant, Serum (100 ul)

Sensitivity

20 pg/ml

Reactivity

Human

Product Specifications

Description

Sandwich ELISA for the quantitative determination of Human IL-38/IL-1F10

Sample Volume Required

100 ul

Conjugate

Unconjugated

Application Notes

1. Dilute the desired amount of Coating Antibody (COAT) (1 mg/ml) (rabbit polyclonal antibody) to 5 ug/ml in PBS without carrier protein and use it fresh.
2. Dilute the desired amount of Detection Antibody (DET) (1 mg/ml) (mouse monoclonal antibody) to 1 ug/ml in ELISA Buffer and use it fresh.
3. Reconstitute the Anti-IgG-HRP (HRP) with 100 ul ELISA Buffer. After reconstitution, prepare aliquots and store the reconstituted HRP at -20C. Avoid freeze/thaw cycles. Dilute the reconstituted AntiIgG-HRP to the working concentration by adding 10 ul in 10 ml of ELISA Buffer (1:1000).
4. Reconstitute the Standard Protein (STD) (recombinant human IL-38/IL-1F10) with 100 ul ELISA Buffer to obtain a concentration of 10 ug/ml. After reconstitution, prepare aliquots and store the reconstituted standard at -20C. Avoid freeze/ thaw cycles. A standard curve using 2-fold serial dilutions in ELISA Buffer is recommended. Suggested standard points are 2 ng/ml, 1 ng/ml, 0.5 ng/ml, 0.25 ng/ml, 0.125 ng/ml, 0.062 ng/ml, 0.031 ng/ml and 0 ng/ml.

Kit Contents for IL-38/IL-1F10 Antibody Pair

  • 1 vial anti-IgG-HRP (10 ug) (lyophilized) [HRP]
  • 1 vial coating antibody (270 ul) [COAT]
  • 1 vial detection antibody (60 ul) [DET]
  • 1 vial standard protein (1 ug) (lyophilized) [STD]

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-38/IL-1F10

Human IL-38/IL-1F10 is a secreted cytokine belonging to the IL-1 family. It is synthesized as a 152 amino acid mature protein that lacks an identifiable signal sequence. It binds to soluble IL-1 R1. Human IL-38 shares 82% aa sequence identity with mouse IL-1F10. Human IL-38 also shares 39% and 27% aa sequence identity with IL-1ra and IL-1 beta, respectively.

Long Name

Interleukin 1 Family Member 10

Alternate Names

FIL1- theta, FKSG75, IL-1HY2, IL1F10, IL38

Gene Symbol

IL1F10

Additional IL-38/IL-1F10 Products

Product Documents for IL-38/IL-1F10 Antibody Pair

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-38/IL-1F10 Antibody Pair

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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