Human IL-5 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91274

Novus Biologicals, part of Bio-Techne
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NBP1-91274
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (50 uL)

Sensitivity

1.5 pg/mL

Assay Range

7.8 - 500 pg/mL

View all IL-5 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-5 positive serum. There was no crossreactivity detected.

Description

The human IL-5 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-5.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6.60%

Inter-Assay Precision (Precision between assays) CV% < 6.80%

Recovery for Human IL-5 ELISA Kit (Colorimetric)

Recovery

74%

Scientific Data Images for Human IL-5 ELISA Kit (Colorimetric)

Human IL-5 ELISA Kit [NBP1-91274] - Data for Human IL-5 Platinum ELISA

Kit Contents for Human IL-5 ELISA Kit (Colorimetric)

  • Adhesive Plate Covers
  • Aluminium pouch(es) with a human IL-5 Standard curve (coloured)
  • Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to human IL-5, Biotin-Conjugate (anti-IL-5 monoclonal antibody), Streptavidin-HRP and Sample Diluent, lyophilized
  • Sample Diluent (Use when an external predilution of the samples is needed)
  • Stop Solution (1M Phosphoric acid)
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-5

The human IL-5 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-5. Human IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stem cells, induction of mIL-2R expression on activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. IL-5 has been shown to be produced by the TH2 subset of human TH clones. The spectrum of biological activities of IL-5, on B cells as well as other cell types suggests that IL-5 plays an important functional role in the activities of TH2 cells. IL-5 is a heavily glycosylated glycoprotein.

Long Name

Interleukin 5

Alternate Names

BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF

Gene Symbol

IL5

Additional IL-5 Products

Product Documents for Human IL-5 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IL-5 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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