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Human IL-7R alpha/CD127 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06768

Novus Biologicals, part of Bio-Techne
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NBP3-06768

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 uL)

Sensitivity

0.94 ng/mL

Assay Range

1.56 - 100 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human IL-7R alpha/CD127 in samples. No significant cross-reactivity or interference between Human IL-7R alpha/CD127 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.39

Inter-Assay Precision (Precision between assays) %CV < 5.36

Recovery for Human IL-7R alpha/CD127 ELISA Kit (Colorimetric)

Recovery

87 - 111%

Linearity

Human IL-7R alpha/CD127 ELISA Kit (Colorimetric)

Scientific Data Images for Human IL-7R alpha/CD127 ELISA Kit (Colorimetric)

Human IL-7R alpha/CD127 ELISA Kit (Colorimetric)

ELISA: Human IL-7R alpha/CD127 ELISA Kit (Colorimetric) [NBP3-06768] -

ELISA: Human IL-7R alpha/CD127 ELISA Kit (Colorimetric) [NBP3-06768] - Standard Curve Reference

Kit Contents for Human IL-7R alpha/CD127 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-7R alpha/CD127

Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. The human and mouse sequence is nearly identical. In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged.

Long Name

Interleukin 7 Receptor alpha

Alternate Names

CD127, IL-7 R alpha, IL-7Ra, IL7R, IL7R alpha

Gene Symbol

IL7R

Additional IL-7R alpha/CD127 Products

Product Documents for Human IL-7R alpha/CD127 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IL-7R alpha/CD127 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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