Human IL6R ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89870
Key Product Details
Sample Type & Volume Required Per Well
Cell culture supernatant, serum, plasma (EDTA, heparin), amniotic fluid, urine (20 uL)
Sensitivity
0.01 ng/mL
Assay Range
0.08 - 5.0 ng/mL
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Specificity
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-6R positive serum. There was no crossreactivity detected.
Description
This kit is an enzyme-linked immunosorbent assay for quantitative detection.
Precision
Intra-Assay Precision (Precision within an assay) CV% < 1.7%
Inter-Assay Precision (Precision between assays) CV% < 2.2%
Scientific Data Images for Human IL6R ELISA Kit (Colorimetric)
Sandwich ELISA: Human IL6R ELISA Kit (Colorimetric) [NBP1-89870] - Representative standard curve for human IL-6R ELISA. Human IL-6R was diluted in serial 2-fold steps in IL-6RAssay Buffer (1x). Do not use this standard curve to derive test results. A standard curve must be run for each group ofmicrowell strips assayed.
Kit Contents for Human IL6R ELISA Kit (Colorimetric)
- Adhesive Plate Covers
- Aluminium pouch(es) with a human sIL-6R Standard curve (coloured)
- Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to human sIL-6R and HRP-Conjugate (anti-sIL-6R monoclonal (murine) antibody), lyophilized
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Lyophilized Control
- Stop Solution (1M Phosphoric acid)
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: IL6R
Alternate Names
CD126, CD126 antigen, gp80, IL-6 receptor subunit alpha, IL-6R 1, IL-6R subunit alpha, IL-6R-1, IL6RA, IL-6RA, IL-6R-alpha, interleukin 6 receptor, interleukin 6 receptor alpha subunit, interleukin-6 receptor subunit alpha, Membrane glycoprotein 80, MGC104991
Gene Symbol
IL6R
Additional IL6R Products
Product Documents for Human IL6R ELISA Kit (Colorimetric)
Product Specific Notices for Human IL6R ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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