Rat iNOS ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80257
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 ul)
Sensitivity
0.188 ng/mL
Assay Range
0.313-20 ng/mL
Product Specifications
Assay Type
Sandwich-ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Rat
Specificity
This kit recognizes Rat NOS2/iNOS in samples. No significant cross-reactivity or interference between Rat NOS2/iNOS and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) CV% < 6.21%
Inter-Assay Precision (Precision between assays) CV% < 5.99%
Recovery for Rat iNOS ELISA Kit (Colorimetric)
Recovery
86-102%
Linearity
Scientific Data Images for Rat iNOS ELISA Kit (Colorimetric)
ELISA: Rat iNOS ELISA Kit (Colorimetric) [NBP2-80257] -
ELISA: Rat iNOS ELISA Kit (Colorimetric) [NBP2-80257] - Standard Curve ReferenceKit Contents for Rat iNOS ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100X)
- Concentrated HRP Conjugate (100X)
- Concentrated Wash Buffer (25X)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: iNOS
The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).
References
1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489
2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174
3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483
Long Name
Inducible Nitic Oxide Synthase
Alternate Names
NOS2, NOS2A
Gene Symbol
NOS2
Additional iNOS Products
Product Documents for Rat iNOS ELISA Kit (Colorimetric)
Product Specific Notices for Rat iNOS ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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