Mouse Lactate Dehydrogenase B ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-42468

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Tissue homogenates and other biological fluids (100 uL)

Sensitivity

0.064 ng/mL

Assay Range

0.156 - 10 ng/mL

View all Lactate Dehydrogenase B/LDHB ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Description

Assay time: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10

Inter-Assay Precision (Precision between assays) %CV < 12

Recovery for Mouse Lactate Dehydrogenase B ELISA Kit (Colorimetric)

Recovery

80 - 98%

Kit Contents for Mouse Lactate Dehydrogenase B ELISA Kit (Colorimetric)

  • Assay Diluent A
  • Assay Diluent B
  • Detection Reagent A
  • Detection Reagent B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated ready to use 96-well strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Lactate Dehydrogenase B/LDHB

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140 kDa). Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor (2).

Alternate Names

Epididymis Secretory Protein Li 281, HEL-S-281, LDH-H, LDHB, LDHBD, Renal Carcinoma Antigen NY-REN-46, TRG-5

Gene Symbol

LDHB

Additional Lactate Dehydrogenase B/LDHB Products

Product Documents for Mouse Lactate Dehydrogenase B ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse Lactate Dehydrogenase B ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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