Human Lactoperoxidase ELISA Kit (Colorimetric)

Name: Human Lactoperoxidase ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-39740

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-39740

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Breast milk, tears and other biological fluids (100 uL)

Sensitivity

0.057 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Lactoperoxidase ELISA Kit (Colorimetric)

ELISA: Human Lactoperoxidase ELISA Kit (Colorimetric) [NBP3-39740]

ELISA: Human Lactoperoxidase ELISA Kit (Colorimetric) [NBP3-39740] - Standard Curve Reference

Kit Contents for Human Lactoperoxidase ELISA Kit (Colorimetric)

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Lactoperoxidase

Lactoperoxidase is an antibacterial agent in cow milk. The heme protein lactoperoxidase (LPO), also referred to as salivary peroxidase (SPO), is an oxidoreductase secreted into milk. LPO belongs to the XPO subfamily of the peroxidase family. It is expressed in mammary and salivary glands, and in the presence of H2O2, LPO acts as a catalyst for the oxidation of many phenols and aromatic amines. It is crucial for protecting the lactating mammary gland and intestinal tract of newborn infants against microorganisms. LPO binds one calcium ion per heterodimer and one heme B (iron-protoporphyrin IX) group covalently per heterodimer. The LPO gene, which spans 28 kb, is similar in gene organization and sequence to the peroxidase genes MPO and EPX, suggesting the possibility that these genes evolved from a common ancestral gene. The LPO and MPO genes are arranged in a tail-to-tail manner on chromosome 17q23.1.

Alternate Names

EC 1.11.1, lactoperoxidase, MGC129990, MGC129991, Salivary peroxidase, SAPX, SPOEC 1.11.1.7

Gene Symbol

LPO

Additional Lactoperoxidase Products

Product Documents for Human Lactoperoxidase ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human Lactoperoxidase ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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