Human, Mouse, Rat Leptin/OB ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA0026

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Plasma (Citrate, EDTA, Heparin), Serum (100 uL)

Sensitivity

30 pg/mL (mouse), 50 pg/mL (rat)

Assay Range

100 to 4000 pg/mL

View all Leptin/OB ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human, Mouse, Rat

Description

Quality control test: Standard curve. Lep (Mouse/Rat) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse and/or rat leptin in serum, plasma.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 2.20

Inter-Assay Precision (Precision between assays) %CV < 3.40

Recovery for Human, Mouse, Rat Leptin/OB ELISA Kit (Colorimetric)

Recovery

94.50%

Scientific Data Images for Human, Mouse, Rat Leptin/OB ELISA Kit (Colorimetric)

ELISA: Human, Mouse, Rat Leptin/OB ELISA Kit (Colorimetric) [KA0026]

ELISA: Human, Mouse, Rat Leptin/OB ELISA Kit (Colorimetric) [KA0026]

ELISA: Leptin/OB ELISA Kit [HRP] [KA0026] - Standard curve

Kit Contents for Human, Mouse, Rat Leptin/OB ELISA Kit (Colorimetric)

  • Antibody Coated Microtiter Strips (Ready to use)
  • Biotin Labelled Antibody Conc.(10x)
  • Biotin–Ab Diluent (Ready to use)
  • Dilution Buffer (Ready to use)
  • Master Standard Mouse (lyophilized)
  • Master Standard Rat (lyophilized)
  • Quality Control Mouse (lyophilized)
  • Quality Control Rat (lyophilized)
  • Stop Solution (Ready to use)
  • Streptavidin-HRP Conjugate (Ready to use)
  • Substrate Solution (Ready to use)
  • Wash Solution Conc. (10x)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Leptin/OB

Leptin, the obese (ob) gene product, is a small protein expressed in and secreted from adipose tissue of normal rodents. Studies suggest leptin acts as a circulating hormone capable of regulating body-weight homeostasis and energy balance. One possible target tissue for leptin is the hypothalamus, a proposed control center for satiety and energy expenditure. Ob gene knockout mice are characterized by several metabolic abnormalities including hyperglucocorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered central nervous system activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue. Studies show that the administration of recombinant leptin to ob knockout mice reduces food intake and increases energy expenditure.

Alternate Names

LEP, OB

Gene Symbol

LEP

Additional Leptin/OB Products

Product Documents for Human, Mouse, Rat Leptin/OB ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human, Mouse, Rat Leptin/OB ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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