Human Luteinizing Hormone ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60087

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-60087-1Kit
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

0.5 mIU/mL

Assay Range

1 mIU/mL - 50 mIU/mL

View all Luteinizing Hormone ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The minimum detectable dose of human LH is typically less than 0.5 mIU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human LH concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 15

Inter-Assay Precision (Precision between assays) %CV < 15

Scientific Data Images for Human Luteinizing Hormone ELISA Kit (Colorimetric)

Human Luteinizing Hormone ELISA Kit (Colorimetric)

Human Luteinizing Hormone ELISA Kit (Colorimetric) [NBP2-60087] -

Human Luteinizing Hormone ELISA Kit (Colorimetric) [NBP2-60087] - Standard Curve Reference

Kit Contents for Human Luteinizing Hormone ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate
  • Instruction manual
  • Standard
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer (20 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Luteinizing Hormone

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for LH. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Alternate Names

CGB4;HH23;LHB;LSH-B;LSH-beta;Lutropin subunit beta

Gene Symbol

LHB

Additional Luteinizing Hormone Products

Product Documents for Human Luteinizing Hormone ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Luteinizing Hormone ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.