Human MAP2 ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80250
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 ul)
Sensitivity
0.188 ng/mL
Assay Range
0.313-20 ng/mL
Product Specifications
Assay Type
Sandwich-ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Specificity
This kit recognizes Human MAP-2 in samples. No significant cross-reactivity or interference between Human MAP-2 and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) CV% < 5.54%
Inter-Assay Precision (Precision between assays) CV% < 5.62%
Recovery for Human MAP2 ELISA Kit (Colorimetric)
Recovery
86-106%
Linearity
Scientific Data Images for Human MAP2 ELISA Kit (Colorimetric)
ELISA: Human MAP2 ELISA Kit (Colorimetric) [NBP2-80250] -
ELISA: Human MAP2 ELISA Kit (Colorimetric) [NBP2-80250] - Standard Curve ReferenceKit Contents for Human MAP2 ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100X)
- Concentrated HRP Conjugate (100X)
- Concentrated Wash Buffer (25X)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: MAP2
MAP2 isoforms are developmentally regulated and differentially expressed in neurons and some glia. MAP2c is predominantly expressed in the developing brain while the other isoforms are expressed in the adult brain. The distribution of MAP2 isoforms also varies, with MAP2a and MAP2b predominantly localized to dendrites, while MAP2c is also found in axons. Lastly, the expression of MAP2d is not limited to neurons and may be found in glia, specifically oligodendrocytes (1, 2). MAP2 isoforms associate with microtubules and mediate their interaction with actin filaments thereby playing a critical role in organizing the microtubule-actin network. In neurons, MAP2 isoforms are implicated in different processes including neurite initiation, elongation and stabilization as well as axon and dendrite formation (2). Knockout of MAP expression in animal models results in a variety of functional and structural brain defects according to the isoform affected (e.g., reduced LTP and LTD, reduced myelination, absence of corpus collosum, motor system malfunction, abnormal hippocampal dendritic morphology, abnormal synaptic plasticity) (4).
References
1. Dehmelt, L., & Halpain, S. (2005). The MAP2/Tau family of microtubule-associated proteins. Genome Biology. https://doi.org/10.1186/gb-2004-6-1-204
2. Mohan, R., & John, A. (2015). Microtubule-associated proteins as direct crosslinkers of actin filaments and microtubules. IUBMB Life. https://doi.org/10.1002/iub.1384
3. Shafit-Zagardo, B., & Kalcheva, N. (1998). Making sense of the multiple MAP-2 transcripts and their role in the neuron. Molecular Neurobiology. https://doi.org/10.1007/BF02740642
4. Tortosa, E., Kapitein, L. C., & Hoogenraad, C. C. (2016). Microtubule organization and microtubule-associated proteins (MAPs). In Dendrites: Development and Disease. https://doi.org/10.1007/978-4-431-56050-0_3
Long Name
Microtubule-associated Protein 2
Alternate Names
DKFZp686I2148, MAP-2, MAP2A, MAP2B, MAP2C, Microtubule Associated Protein 2, microtubule-associated protein 2, MTAP2
Gene Symbol
MAP2
UniProt
Additional MAP2 Products
Product Documents for Human MAP2 ELISA Kit (Colorimetric)
Product Specific Notices for Human MAP2 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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