Human MELK - Ready-To-Use ELISA Kit (Colorimetric)

Name: Human MELK - Ready-To-Use ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-39806

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-39806

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.105 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human MELK - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human MELK - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39806]

ELISA: Human MELK - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39806] - Standard Curve Reference

Kit Contents for Human MELK - Ready-To-Use ELISA Kit (Colorimetric)

Detection Solution A
Detection Solution B
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Standard Diluent
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MELK

MELK is a member of AMPK family, which has several consensus glycosylation sites. Glycosylations are known to affect migration of proteins on a SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press]. Further the activity of this antibody was confirmed against recombinant, GST fused MELK protein by Western blotting.

Long Name

Maternal Embryonic Leucine Zipper Kinase

Alternate Names

PK38

Gene Symbol

MELK

Additional MELK Products

Product Documents for Human MELK - Ready-To-Use ELISA Kit (Colorimetric)

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for Human MELK - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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