Human MEPE/OF45 - Ready-To-Use ELISA Kit (Colorimetric)

Name: Human MEPE/OF45 - Ready-To-Use ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-39810

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-39810

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.29 ng/mL (example only; lot dependent)

Assay Range

0.937 - 60 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human MEPE/OF45 - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human MEPE/OF45 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39810]

ELISA: Human MEPE/OF45 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39810] - Standard Curve Reference

Kit Contents for Human MEPE/OF45 - Ready-To-Use ELISA Kit (Colorimetric)

Detection Solution A
Detection Solution B
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Standard Diluent
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MEPE/OF45

MEPE, known as OF45 in mouse and rat, is a 55 kDa member of the SIBLING protein family that is primarily expressed in bone and dentin where it regulates the mineralization of those tissues. MEPE is secreted by osteoblasts and dental pulp stem cells during the mineralization process and also by non-mineralizing tissues including epithelial cells in the renal proximal tubule and salivary duct. MEPE contains multiple consensus sites for post-translational modifications, including N-linked glycosylation, N-myristoylation, glycosaminoglycan attachment, and phosphorylation by a variety of kinases. MEPE also contains several putative proteolytic cleavage sites and one integrin-binding RGD motif.

Long Name

Matrix Extracellular Phosphoglycoprotein with ASARM Motif

Alternate Names

OF45, Osteoregulin

Gene Symbol

MEPE

Additional MEPE/OF45 Products

Product Documents for Human MEPE/OF45 - Ready-To-Use ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human MEPE/OF45 - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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