Human Midkine ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66744

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25-2000 pg/mL

View all Midkine ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human MK in samples. No significant cross-reactivity or interference between Human MK and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 9.59%

Inter-Assay Precision (Precision between assays) CV% < 9.97%

Recovery for Human Midkine ELISA Kit (Chemiluminescence)

Recovery

90-111%

Linearity

Human Midkine ELISA Kit (Chemiluminescence)

Scientific Data Images for Human Midkine ELISA Kit (Chemiluminescence)

Human Midkine ELISA Kit (Chemiluminescence)

ELISA: Human Midkine ELISA Kit (Chemiluminescence) [NBP2-66744] -

ELISA: Human Midkine ELISA Kit (Chemiluminescence) [NBP2-66744] - Standard Curve Reference

Kit Contents for Human Midkine ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Midkine

Midkine (MK) is a 13 kDa heparin-binding polypeptide which enhances neurite outgrowth, neuronal cell survival and plasminogen activator activity. MK is structurally divided into two domains, and most of the biological activities are located on the C-terminal domain. Both domains consist of three antiparallel beta-strands, but the C-terminal domain has a long flexible hairpin loop where a heparin-binding consensus sequence is located (1). MK is a member of a highly conserved, developmentally regulated human gene family. The gene product exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Expression of MK is predominant only for a short period from approximately one-half to two-thirds of the way through gestation; before and after that, it is barely detectable (2)

Alternate Names

ARAP, MDK, MEK, MK1, NEGF2

Gene Symbol

MDK

Additional Midkine Products

Product Documents for Human Midkine ELISA Kit (Chemiluminescence)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Midkine ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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