Human MKP-1/DUSP1 - Ready-To-Use ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-31428

Novus Biologicals, part of Bio-Techne
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NBP3-31428
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and other biological fluids (100 uL)

Sensitivity

12.9 pg/mL (example only; lot dependent)

Assay Range

31.2 - 2000 pg/mL (example only; lot dependent)

View all MKP-1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human MKP-1/DUSP1 - Ready-To-Use ELISA Kit (Colorimetric)

Human MKP-1/DUSP1 - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human MKP-1/DUSP1  - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31428] -

ELISA: Human MKP-1/DUSP1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31428] - Standard Curve Reference

Kit Contents for Human MKP-1/DUSP1 - Ready-To-Use ELISA Kit (Colorimetric)

  • Detection Solution A
  • Detection Solution B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MKP-1

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.

Long Name

MAP Kinase Phosphatase 1

Alternate Names

CL100, DUSP1, HVH1, MKP1, PTPN10

Gene Symbol

DUSP1

Additional MKP-1 Products

Product Documents for Human MKP-1/DUSP1 - Ready-To-Use ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human MKP-1/DUSP1 - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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