Human MMP-12 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06762

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

View all MMP-12 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human MMP-12 in samples. No significant cross-reactivity or interference between Human MMP-12 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 5.33

Inter-Assay Precision (Precision between assays) 5.13

Recovery for Human MMP-12 ELISA Kit (Colorimetric)

Recovery

92 - 109 %

Linearity

Human MMP-12 ELISA Kit (Colorimetric)

Scientific Data Images for Human MMP-12 ELISA Kit (Colorimetric)

Human MMP-12 ELISA Kit (Colorimetric)

ELISA: Human MMP-12 ELISA Kit (Colorimetric) [NBP3-06762] -

ELISA: Human MMP-12 ELISA Kit (Colorimetric) [NBP3-06762] - Standard Curve Reference

Kit Contents for Human MMP-12 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MMP-12

Human macrophage elastase (MMP-12) is a member of the family of matrix metalloproteinases (MMPs) that plays, like other members of the family, an important role in inflammatory processes contributing to tissue remodeling and destruction. In particular, a prominent role of MMP-12 in the destruction of elastin in the lung alveolar wall and the pathogenesis of emphysema has been suggested. It is therefore an attractive therapeutic target (1). MMP-12) expressed mainly in alveolar macrophages has been identified in the mouse lung as the main destructive agent associated with cigarette smoking, which gives rise to emphysema, both directly via elastin degradation and indirectly by disturbing the proteinase/antiproteinase balance via inactivation of the alpha1-proteinase inhibitor (alpha1-PI), the antagonist of the leukocyte elastase (2). Because elastin represents a critical component of aortic wall structure and a matrix substrate for metalloelastases, MMP-12 may have a direct and singular role in the pathogenesis of aortic aneurysms (3).

Long Name

Matrix Metalloproteinase 12

Alternate Names

MMP12

Gene Symbol

MMP12

Additional MMP-12 Products

Product Documents for Human MMP-12 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human MMP-12 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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